IN VITRO EFFECT OF NEWLY BACTERIOPHAGES ISOLATES AGAINST ESCHERICHIA COLI SEROGROUPS COLLECTED FROM THE LOCAL HOSPITAL LABORATORY; THEIR SPECIFIC CHARACTERIZATION AND IDENTIFICATION

The aim of this study is to isolate a new bacteriophage with lytic effect against several serogroups Escherichia coli that were isolated from different samples submitted the local hospital laboratory. These are main causative agents bloodstream and urinary tract infections (UTIs) among Gram-negative bacteria. Three isolates E. used as host phages wastewater treatment plant. Eight only three them (EC-BSR1, EC-BSR2, EC-BSR3) considered for further characterization identification. appear belong Myoviridae characterized by icosahedral heads, necks contractile tails tail fibers. Phage EC-BSR1 EC-BSR2 had genome sizes 67.06 68.04 kb, respectively. All lysed 100% was tested in vitro 42.7%, 61.7% 44.7% lyses ECOR collection reference. Phages resistant pH 5 9, phage EC-BSR3 appeared be more than other two an acidic alkaline environment. have pattern homologous DNA fragments after being digested Acc1 EcoR1 restriction enzymes. It concluded these highly isolates.